High-throughput analysis of CB formation. (A) Dot plot showing the relationship between coilin concentration and CB formation under different temperature. Y axis show coilin concentration per cell (represented by average intensity) or total number of CBs present per cell (Number of bodies); X axis represent either CBs total area (Body area) or total amount of coilin present in the CBs per cell (body intensity). Dashed red lines are linear fitting of the data and R² the coefficient of determination. (B) Histogram of number of bodies per cell distribution when cells are incubated at different temperature. The data represent the mean of three independent experiments, and error bars represent standard deviation.