Figure S2.
B cells activated on stiff substrates display enhanced BCR signaling and microtubule acetylation. (A and B) Top: Western blot of B cells activated on soft and stiff surfaces for different time points to measure pAKT, pERK, and GAPDH (A), or Ac-Tub (B). Bottom: Quantifications of the relative abundance of pAKT and pERK with respect to GAPDH (A) or Ac-Tub with respect to total tubulin (B). (C) Western blot analysis of Ac-Tub levels in B cells treated with SAHA. Representative immunoblots showing the expression of Ac-Tub, total α-Tub, and GAPDH as a loading control in vehicle (DMSO) and SAHA-treated B cells. The quantification (right) represents the ratio of Ac-Tub to total α-Tub, normalized to the DMSO condition. t test. P values illustrated with asterisks are * <0.05. Source data are available for this figure: SourceData FS2. Refer to the image caption for details.

B cells activated on stiff substrates display enhanced BCR signaling and microtubule acetylation. (A and B) Top: Western blot of B cells activated on soft and stiff surfaces for different time points to measure pAKT, pERK, and GAPDH (A), or Ac-Tub (B). Bottom: Quantifications of the relative abundance of pAKT and pERK with respect to GAPDH (A) or Ac-Tub with respect to total tubulin (B). (C) Western blot analysis of Ac-Tub levels in B cells treated with SAHA. Representative immunoblots showing the expression of Ac-Tub, total α-Tub, and GAPDH as a loading control in vehicle (DMSO) and SAHA-treated B cells. The quantification (right) represents the ratio of Ac-Tub to total α-Tub, normalized to the DMSO condition. t test. P values illustrated with asterisks are * <0.05. Source data are available for this figure: SourceData FS2.

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