Figure 5.
Decreased MCA promotes invadosome formation for cell–cell fusion. (A) Confocal images of parental cells and stable cell lines expressing ezrin, MA-ezrin (MA), and iMC-linker (iMC), without or with RANKL treatment for 72 h, stained with phalloidin (gray) and DAPI (blue). Arrowheads indicate mononuclear cells forming invadosomes. Scale bar: 10 μm. (B) Quantification of the percentage of mononuclear cells forming invadosomes in A. Mean ± SEM, n = 15 fields of view for each condition. P value obtained from one-way ANOVA with Dunnett’s test. ns: not significant, ****P < 0.0001. (C) Confocal images of ezrin and ERM knockdown cells treated with RANKL for 60 h, stained with phalloidin (gray) and DAPI (blue). Arrowheads indicate mononuclear cells forming invadosomes. Scale bar: 10 μm. (D) Quantification of (C). Mean ± SEM, n = 15 fields of view for each condition. P value obtained from one-way ANOVA with Dunnett’s test. ***P < 0.001; ****P < 0.0001. (E) Confocal images of Baiap2l1 and Fnbp1 knockdown cells treated with RANKL for 72 h, stained with phalloidin (gray) and DAPI (blue). Arrowheads indicate mononuclear cells forming invadosomes. Scale bar: 10 μm. (F) Quantification of (E). Mean ± SEM, n = 15 fields of view for each condition. P value obtained from one-way ANOVA with Dunnett’s test. **P < 0.01; ***P < 0.001. (G) Confocal image of a stable cell line expressing GFP-IRTKS stained with anti-GFP antibody (green) and phalloidin (magenta) after ERM knockdown and RANKL treatment for 72 h. Scale bar: 10 μm. (H) Confocal image of a stable cell line expressing GFP-FBP17 stained with anti-FBP17 antibody (green) and phalloidin (magenta) after ERM knockdown and RANKL treatment for 72 h. Scale bar: 10 μm.

Decreased MCA promotes invadosome formation for cell–cell fusion. (A) Confocal images of parental cells and stable cell lines expressing ezrin, MA-ezrin (MA), and iMC-linker (iMC), without or with RANKL treatment for 72 h, stained with phalloidin (gray) and DAPI (blue). Arrowheads indicate mononuclear cells forming invadosomes. Scale bar: 10 μm. (B) Quantification of the percentage of mononuclear cells forming invadosomes in A. Mean ± SEM, n = 15 fields of view for each condition. P value obtained from one-way ANOVA with Dunnett’s test. ns: not significant, ****P < 0.0001. (C) Confocal images of ezrin and ERM knockdown cells treated with RANKL for 60 h, stained with phalloidin (gray) and DAPI (blue). Arrowheads indicate mononuclear cells forming invadosomes. Scale bar: 10 μm. (D) Quantification of (C). Mean ± SEM, n = 15 fields of view for each condition. P value obtained from one-way ANOVA with Dunnett’s test. ***P < 0.001; ****P < 0.0001. (E) Confocal images of Baiap2l1 and Fnbp1 knockdown cells treated with RANKL for 72 h, stained with phalloidin (gray) and DAPI (blue). Arrowheads indicate mononuclear cells forming invadosomes. Scale bar: 10 μm. (F) Quantification of (E). Mean ± SEM, n = 15 fields of view for each condition. P value obtained from one-way ANOVA with Dunnett’s test. **P < 0.01; ***P < 0.001. (G) Confocal image of a stable cell line expressing GFP-IRTKS stained with anti-GFP antibody (green) and phalloidin (magenta) after ERM knockdown and RANKL treatment for 72 h. Scale bar: 10 μm. (H) Confocal image of a stable cell line expressing GFP-FBP17 stained with anti-FBP17 antibody (green) and phalloidin (magenta) after ERM knockdown and RANKL treatment for 72 h. Scale bar: 10 μm.

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