Figure 4.
Knockdown of Baiap2, Baiap2l1, and the TOCA family proteins inhibits cell–cell fusion by RANKL-induced osteoclastogenesis. (A) The fusion index after treatment with the indicated RNAi targeting BAR proteins. Dotted lines in violin plots show median and quantiles. The total number of fields of view analyzed was as follows: n = 85 (si-Control), n = 80 (si-DC-STAMP), and n = 45 (si-BAR for each condition). P value obtained from one-way ANOVA with Dunnett’s test. *P < 0.05; **P < 0.01; ****P < 0.0001. (B) Confocal images of siRNA knockdown cells treated with RANKL for 84 h, stained with phalloidin (magenta) and DRAQ5 (gray). Multinucleated cells are surrounded by dotted lines. Scale bar: 50 μm. (C) siRNA knockdown cells stained for TRAP activity. Multinucleated cells are surrounded by dotted lines. Note that siRNA knockdown cells treated with RANKL for 84 h indicate the activity of TRAP. Scale bar: 100 μm.

Knockdown of Baiap2, Baiap2l1, and the TOCA family proteins inhibits cell–cell fusion by RANKL-induced osteoclastogenesis. (A) The fusion index after treatment with the indicated RNAi targeting BAR proteins. Dotted lines in violin plots show median and quantiles. The total number of fields of view analyzed was as follows: n = 85 (si-Control), n = 80 (si-DC-STAMP), and n = 45 (si-BAR for each condition). P value obtained from one-way ANOVA with Dunnett’s test. *P < 0.05; **P < 0.01; ****P < 0.0001. (B) Confocal images of siRNA knockdown cells treated with RANKL for 84 h, stained with phalloidin (magenta) and DRAQ5 (gray). Multinucleated cells are surrounded by dotted lines. Scale bar: 50 μm. (C) siRNA knockdown cells stained for TRAP activity. Multinucleated cells are surrounded by dotted lines. Note that siRNA knockdown cells treated with RANKL for 84 h indicate the activity of TRAP. Scale bar: 100 μm.

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