Ectopic expression of LET-92 affects OSM-3’s motility. (A) Ciliary localization of mScarlet-tagged LET-92 protein in OSM-3::GFP knock-in animals. Arrows indicate the ciliary base. Arrowheads indicate the junctions between the middle and distal ciliary segments. Scale bars, 5 μm. (B) Schematic of plasmids used to ectopically express fused let-92 at transition zone (mksr-2::let-92) or within cilia (pcrg-1::let-92) under the control of Pdyf-1.(C) Representative fluorescence images of the cilia in the animals ectopically expressing let-92 within cilia. Arrows indicate the ciliary base. Arrowheads indicate the junctions between the middle and distal ciliary segments. Scale bar, 5 μm. (D) Histogram of OSM-3::GFP velocities in animals of indicated genotypes. (Top) anterograde IFT along the middle segments (Antero. m.s.). (Middle) anterograde IFT along the distal segments (Antero. d.s.). (Bottom) retrograde IFT (Retro.). Each plot was fitted by a Gaussian distribution. Comparisons were performed between mksr-2::let-92^H127G and other strains. ***P < 0.001 by unpaired Student’s t tests. m.s.: middle segment; d.s.: distal segment.