Loss of NEKL-3/4 affects regional motility of OSM-3 in sensory cilia. (A) Representative fluorescence intensity profiles along the cilium. All fluorescence intensity profiles are normalized to their maximum. N = 15–19 animals. Data are mean ± SD. (B) Kymographs showing the anterograde movement of OSM-3::GFP in the middle segments and distal segments. Representative particle traces are marked with green, purple, yellow, and red lines. The scale bars represent 5 μm (horizontal) and 20 s (vertical). (C) Histogram of OSM-3::GFP velocities. (Top) anterograde IFT along the middle segments (Antero. m.s.). (Middle) anterograde IFT along the distal segments (Antero. d.s.). (Bottom) retrograde IFT (Retro.). Each plot was fitted by a Gaussian distribution. Velocities and numbers of IFT particles (N) are indicated. Comparisons were performed between WT and other strains. *P < 0.05; **P < 0.01; ***P < 0.001 by unpaired Student’s t tests. m.s.: middle segment; d.s.: distal segment.