NEKL-3 and NEKL-4 kinases regulate OSM-3’s regional motility. (A) Heatmap showing the expression levels of nekl-3, nekl-4, and let-92 in ciliated sensory neurons. The plot was generated from CeNGEN website (https://cengen.shinyapps.io/CengenApp/). The size of each circle corresponds to the proportion of neurons in each cluster expressing a particular gene. TPM, transcripts per million. (B) Distribution of OSM-3::GFP within the amphid cilia of nekl-3 and nekl-4 mutant animals. Scale bar, 5 μm. (C) Statistics of the total fluorescence intensity of OSM-3 labeled with GFP in cilia of the indicated animals. Comparisons were performed between WT and other strains. n.s., not significant by unpaired Student’s t tests. Data are mean ± SD. (D) IFT velocities summary of OSM-3::GFP. Numbers of IFT particles are indicated. Comparisons were performed between WT and other strains. n.s., not significant; ***P < 0.001 by unpaired Student’s t tests. m.s.: middle segment; d.s.: distal segment.