Microtubules and dyneins direct cell shape remodeling around the centrosome. (A) Images show the microtubule network (gray) and the centrosome (green) in PtK2 cells not expressing p150–DN–GFP (top) or expressing p150–DN–GFP (bottom). (B) Live images of the microtubule network (gray) and the centrosome (green) in PtK2 cytoplasts, expressing (bottom) or not (top) p150–DN–GFP, a few hours after the total washout of the enucleation drugs. In A and B, images are max projections further processed using an unsharp mask, an enhance contrast filter, a gamma filter, and a background subtraction filter. (C) Selected time points of representative live centrosome recentering events showing the repositioning of the centrosome and the centroid over a time course of a few hours after the total washout of the enucleation drugs. Images show the recentering in a PtK2 cytoplast not expressing p150–DN–GFP (top), or expressing p150–DN–GFP (bottom). The dotted green line underscores the motion of the centrosome, and the dotted white line underscores the motion of the centroid. Images were processed following the described pipeline in the dedicated Materials and methods section. (D) Vectorial plots depicting the displacement of the centroid toward the centrosome (left), graph representing the magnitude of the displacement of the centroid (right) in the three different centrosome recentering conditions: (1) Nocodazole washout, Y27632 washout, Cytochalasin D washout (n = 70); (2) Nocodazole present, Y27632 washout, Cytochalasin D washout (n = 81), (3) expression of p150–DN–GFP construct and Nocodazole washout, Y27632 washout, Cytochalasin D washout (n = 67). (E) Graph shows the cosine of the angle between the vectors connecting the initial positions of the centrosome and the centroid, and the trajectory of the centroid in the three different centrosome recentering conditions. In D and E, horizontal bars represent the mean. P represents the P values, which were obtained from Kruskal–Wallis non-parametric tests. (F) Schematic depiction of the centrosomal aster and its associated dynein molecular motors guiding the repositioning of the cell body around the centrosome.