Cell shape remodeling around the centrosome. Centrosome recentering in the control condition (Nocodazole washout, Y27632 washout, Cytochalasin D washout) in PtK2 cytoplasts expressing GFP-tubulin (n = 70). (A) Five selected time points of a representative live centrosome recentering event showing the repositioning of the centrosome and the centroid inside a cytoplast over a time course of a few hours. The dotted green line underscores the motion of the centrosome, and the dotted magenta line underscores the motion of the centroid. (B) The left image shows the overlay of the first and last time points of the recentering event depicted in A. The arrows represent the magnitude and direction of the centrosome and the centroid total displacements. The error corresponds to the final distance between the centrosome and the centroid. (C) The left graph shows the average relative contributions of the displacements of the centrosomes and the centroid to the recentering events upon the washout of all the drugs. The right graph shows the relative distance measurements in 80 individual recentering events sorted by final error. Distances are normalized to the initial distance separating the centrosome and the centroid. (D) The graph shows the correlation between centrosome displacement and the distance error. Distances are normalized to the initial distance separating the centrosome and the centroid. (dark green line represents the best linear fit, dotted green line represents the fit’s 95% confidence interval). (E) Same as D but showing the correlation between the displacement of the centroid and the distance error. (F) Example of a centrosome recentering event. Here, recentering occurs almost exclusively through the repositioning of the centroid inside the cytoplast over a time course of a few hours. The dotted green line underscores the motion of the centrosome, and the dotted magenta line underscores the displacement of the centroid. (A and F) Images were processed following the described pipeline in the dedicated Materials and methods section.