MpD forms a right-left gradient along M1. SR-SIM images of cells expressing MpD-GFP (green, detected by polyclonal anti-GFP antibodies) in the native locus, stained with the anti-centrin (magenta) and DAPI (blue). (A and A′) Interphase. Note MpD-GFP at the apical cell end and along M1. The region marked with a star often binds antibodies nonspecifically. (B and B′) Stage 1b. (C and C′) Stage 2. (D and D′) Stage 4a. (E and E′) Late stage 4b. (F and F′) An intermediate between stage 4b and 5a. (G and G′) Early stage 5f. (H and H′) Late stage 5f. Note the weak presence of MpD-GFP in the new M2 and M3. M(1–3), parental M rows; nM(1–3), new M rows; oa, old oral apparatus; noa, new oral apparatus or OP; ac, parental apical crown; nac, new apical crown; cs, cortical subdivision.