Figure 4. Loss of cyclin B uncouples the... | Rockefeller University Press

Figure 4.

Loss of cyclin B uncouples the normal regulation of the G2-M kinase network. (A) Dysregulated phosphorylation and expression of G2–M regulators in the absence of cyclin B. mAIDB1KOB1B2 cells synchronized using double thymidine block were released into a drug-free or DI-containing medium and harvested at different time points for immunoblotting. The positions of the three isoforms of Aurora kinases are indicated. (B) Dysregulation of CDK1 substrate phosphorylation in the absence of cyclin B. Samples prepared from A were immunoblotted with an antibody against CDK1 phosphorylation substrates (pTPxK). The positions of bands that are absent in DI-treated cells are indicated with asterisks. Bands present in both DI-treated and untreated cells but lacking cell cycle variation in DI-treated samples are indicated with circles. Source data are available for this figure: SourceData F4.

Loss of cyclin B uncouples the normal regulation of the G ₂ -M kinase network. (A) Dysregulated phosphorylation and expression of G₂–M regulators in the absence of cyclin B. ^mAIDB1^KOB1B2 cells synchronized using double thymidine block were released into a drug-free or DI-containing medium and harvested at different time points for immunoblotting. The positions of the three isoforms of Aurora kinases are indicated. (B) Dysregulation of CDK1 substrate phosphorylation in the absence of cyclin B. Samples prepared from A were immunoblotted with an antibody against CDK1 phosphorylation substrates (pTPxK). The positions of bands that are absent in DI-treated cells are indicated with asterisks. Bands present in both DI-treated and untreated cells but lacking cell cycle variation in DI-treated samples are indicated with circles. Source data are available for this figure: SourceData F4.