Figure S5.

Site-specific proteomics data of ubiquitin conjugated to PML. Related to Fig. 7, A and B. (A) PML schematic showing positions of lysines modified by ubiquitin. Circle size approximates peptide intensity, and pale circles were not detected in WT-PML purifications. (B) Column charts of average intensity values for the branched peptides diagnostic of PML ubiquitination at the indicated sites. Error bars show the SEM. Number of purifications, n = 4. (C)t test summary for the two-tailed pairwise comparisons indicated for each ubiquitin-PML modification. Welch’s correction was applied for heteroskedastic data. Red entries are P < 0.05. Schematic shows sites in relation to PML sequence and functional domains. The number of different peptides used for each site is indicated (evidence count).

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