Figure 3.
A216T and L217F PML variants show altered SUMO recruitment to PML bodies upon arsenic exposure. (A) Representative images of colocalization of SUMO1 and SUMO2/3 with WT, A216T, and L217F PML variants after 1 μM arsenic treatment for 2 h studied by fluorescence microscopy. Merge shows DAPI (blue), YFP (green), and SUMO (red). (B) Quantitative summary of SUMO1 and SUMO2/3 colocalization with YFP-PML. Columns are average values, and error bars are SEM. Colocalization was defined as any SUMO signal above background in the same region as a PML body. Statistical significance was assessed by comparing the arsenic-treated conditions among the three PML types using Dunn’s multiple comparisons tests after Kruskal–Wallis ANOVA. The number of cells counted (n) is indicated in the charts. Source data are available for this figure: SourceData F3.

A216T and L217F PML variants show altered SUMO recruitment to PML bodies upon arsenic exposure. (A) Representative images of colocalization of SUMO1 and SUMO2/3 with WT, A216T, and L217F PML variants after 1 μM arsenic treatment for 2 h studied by fluorescence microscopy. Merge shows DAPI (blue), YFP (green), and SUMO (red). (B) Quantitative summary of SUMO1 and SUMO2/3 colocalization with YFP-PML. Columns are average values, and error bars are SEM. Colocalization was defined as any SUMO signal above background in the same region as a PML body. Statistical significance was assessed by comparing the arsenic-treated conditions among the three PML types using Dunn’s multiple comparisons tests after Kruskal–Wallis ANOVA. The number of cells counted (n) is indicated in the charts. Source data are available for this figure: SourceData F3.

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