Figure 5.
Nanoblocks containing type A/B particles transsynaptically align with potential release sites. (A) Examples show distribution of docked or tethered or partially fused vesicles and PSD nanoblocks projected onto the postsynaptic membrane in synapses. Circles indicate the positions of docked, tethered, or partially fused vesicles projected onto the postsynaptic membrane in intact synapses. (B) A schematic showing the distance d2 between synaptic vesicle (SV) and the PSD nanoblock centroid and distance D between the nanoblock-centroid and the farthest edge. (C) Distribution of the normalized distance d2/D between the experimental or randomized potential release site and nanoblock in intact synapses from cultured neurons. Two-sample Kolmogorov–Smirnov test, n = 106 for experimental vesicles and n = 10,600 for randomized vesicles, D = 0.09613, P = 0.28. (D) Distribution of the normalized distance d2/D between the experimental or randomized potential release site and nanoblock in synaptosomes. Two-sample Kolmogorov–Smirnov test, n = 145 for experimental vesicles and n = 14,500 for randomized vesicles, D = 0.09448, P = 0.15. (E) Examples show adhesion molecule–like densities linking tethered synaptic vesicles to postsynaptic membrane particles and PSD nanoblocks on the postsynaptic membrane (top two panels and bottom left panel). Bottom right panel shows adhesion molecule–like densities aligning with postsynaptic membrane particles and PSD nanoblocks on the postsynaptic membrane, in the absence of a tethered synaptic vesicle. Red arrowheads indicate adhesion molecule–like densities in the cleft. (F) Normalized distance d2/D between potential release site and centroid of different types of nanoblock. Median and 10–90% range are shown in the figure. Kruskal-Wallis ANOVA, n = 20 for type B nanoblock, n = 28 for mixed nanoblock, n = 119 for type A nanoblock, and n = 725 for no A/B nanoblock. P values are shown in the figure. (G) Area of different types of nanoblocks. Median and 10–90% range are shown in the figure. Kruskal-Wallis ANOVA, n = 5 for type B nanoblock, n = 4 for mixed nanoblock, n = 28 for type A nanoblock, and n = 147 for no A/B nanoblock. P values are shown in the figure. Scale bars: (A) 50 nm; (E) 20 nm.

Nanoblocks containing type A/B particles transsynaptically align with potential release sites. (A) Examples show distribution of docked or tethered or partially fused vesicles and PSD nanoblocks projected onto the postsynaptic membrane in synapses. Circles indicate the positions of docked, tethered, or partially fused vesicles projected onto the postsynaptic membrane in intact synapses. (B) A schematic showing the distance d2 between synaptic vesicle (SV) and the PSD nanoblock centroid and distance D between the nanoblock-centroid and the farthest edge. (C) Distribution of the normalized distance d2/D between the experimental or randomized potential release site and nanoblock in intact synapses from cultured neurons. Two-sample Kolmogorov–Smirnov test, n = 106 for experimental vesicles and n = 10,600 for randomized vesicles, D = 0.09613, P = 0.28. (D) Distribution of the normalized distance d2/D between the experimental or randomized potential release site and nanoblock in synaptosomes. Two-sample Kolmogorov–Smirnov test, n = 145 for experimental vesicles and n = 14,500 for randomized vesicles, D = 0.09448, P = 0.15. (E) Examples show adhesion molecule–like densities linking tethered synaptic vesicles to postsynaptic membrane particles and PSD nanoblocks on the postsynaptic membrane (top two panels and bottom left panel). Bottom right panel shows adhesion molecule–like densities aligning with postsynaptic membrane particles and PSD nanoblocks on the postsynaptic membrane, in the absence of a tethered synaptic vesicle. Red arrowheads indicate adhesion molecule–like densities in the cleft. (F) Normalized distance d2/D between potential release site and centroid of different types of nanoblock. Median and 10–90% range are shown in the figure. Kruskal-Wallis ANOVA, n = 20 for type B nanoblock, n = 28 for mixed nanoblock, n = 119 for type A nanoblock, and n = 725 for no A/B nanoblock. P values are shown in the figure. (G) Area of different types of nanoblocks. Median and 10–90% range are shown in the figure. Kruskal-Wallis ANOVA, n = 5 for type B nanoblock, n = 4 for mixed nanoblock, n = 28 for type A nanoblock, and n = 147 for no A/B nanoblock. P values are shown in the figure. Scale bars: (A) 50 nm; (E) 20 nm.

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