Subtomogram averaging of receptor-like particles in synaptosomes. (A) An example showing receptor-like particles on the postsynaptic membrane of a synaptosome. (B) Processing steps for subtomogram averaging. (C–F) Subtomogram averaged type A particle (C and D) and type B particle (E and F) with AMPAR model fitting. Models from PDB bank are fitted to the averaged electron densities. Yellow: PDB 5IDE. Red: PDB 6QKZ. Scale bars: (A) upper left panel, 100 nm; bottom right panel, 20 nm; (C–F) 5 nm.