Quantification of synaptic components in different sample preparations of synaptosomes. (A) Cleft widths across three preparations (n = 33 for prep 1, n = 18 for prep 2, and n = 23 for prep 3) without protease inhibitor or DTT with mean ± SD of each sample shown in the figure. (B) Synaptic vesicle size distribution for three preparations. (C) Nanoblock height distribution for three preparations. (D) Distribution of PSD nanoblock area, width, centroid-to-centroid distance, and edge-to-edge distance across three preparations. (E) Distribution of PSD nanoblock area, width, centroid-to-centroid distance, and edge-to-edge distance in synaptosomes isolated with protease inhibitor and DTT.