![Tumor-derived sEVs bind much more strongly to laminin than to fibronectin. (A) The diameters of sEVs, mEVs, and MVs derived from the 4175-LuT, PC3, BxPC3, HeLa, and SKBR3 cell lines were measured by qNano. (B) Single-particle fluorescence images of sEV–CD63Halo7-SF650T particles derived from the 4175-LuT, PC3, BxPC3, HeLa, and SKBR3 cell lines on glass coated with fibronectin or laminin. These sEVs were purified by ultracentrifugation (200,000 × g for 4 h). (C–E) The numbers of EV–CD63Halo7-SF650T particles (sEVs [C], mEVs [D], and MVs [E]) attached to glass coated with ECM components (n = 16 images). mEVs and MVs were isolated by low-speed centrifugation (50,000 × g for 30 min) and (10,000 × g for 30 min), respectively. Data are presented as the mean ± SE. The numbers of sEVs, mEVs, and MVs derived from all cell lines applied to laminin- or fibronectin-coated glass were adjusted to the same. (F) Western blotting of integrin subunits in sEVs, mEVs, and MVs derived from 4175-LuT, PC3, BxPC3, HeLa, and SKBR3 cells. Source data are available for this figure: SourceData F3.](https://cdn.rupress.org/rup/content_public/journal/jcb/224/6/10.1083_jcb.202404064/2/jcb_202404064_fig3.png?Expires=1771171984&Signature=ei7ymArzbgVsMNTImCULTtolZN60ATJTDK7tfwNZQ2OcFugSTFX1v8MNg8AGsfzenVw-Z3dv3kPN~VxLvJmqy8I5jF~HIyf5u9WhH5WkgZqHkw4QWUJtHRiAX8l1~5urMzfSMDtcETaoA4Dz6-~jSYFWj9y8GeYiErtL0caYzOg1n3ss1WcF2pGGca6gTaCzTQ-5pAXcI~iRjvgAVk0~E52V-K6gN3wnGF03~IRwijD5o9Sd~qQXaJBUr0X-cVzAHbgfrIArvi~aLNNWE1XbJDT2jorqsmWymmKtrFcI6LNBk9IRGP2oglU0A9saLGj7Xyz-QnXLNuKu8M2UJdeBvw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Tumor-derived sEVs bind much more strongly to laminin than to fibronectin. (A) The diameters of sEVs, mEVs, and MVs derived from the 4175-LuT, PC3, BxPC3, HeLa, and SKBR3 cell lines were measured by qNano. (B) Single-particle fluorescence images of sEV–CD63Halo7-SF650T particles derived from the 4175-LuT, PC3, BxPC3, HeLa, and SKBR3 cell lines on glass coated with fibronectin or laminin. These sEVs were purified by ultracentrifugation (200,000 × g for 4 h). (C–E) The numbers of EV–CD63Halo7-SF650T particles (sEVs [C], mEVs [D], and MVs [E]) attached to glass coated with ECM components (n = 16 images). mEVs and MVs were isolated by low-speed centrifugation (50,000 × g for 30 min) and (10,000 × g for 30 min), respectively. Data are presented as the mean ± SE. The numbers of sEVs, mEVs, and MVs derived from all cell lines applied to laminin- or fibronectin-coated glass were adjusted to the same. (F) Western blotting of integrin subunits in sEVs, mEVs, and MVs derived from 4175-LuT, PC3, BxPC3, HeLa, and SKBR3 cells. Source data are available for this figure: SourceData F3.
