Neither laminin nor fibronectin is present on PC3-derived sEV surfaces. (A) Schematic diagram of the isolation of specific sEVs. sEVs were isolated by ultracentrifugation at 200,000 × g for 4 h (UC sample). Then, special sEVs were isolated from sEVs by a bead-conjugating antibody (IP: immunoprecipitation sample). (B) Western blot analysis of tetraspanin (CD63, CD81, and CD9), fibronectin (FN), and laminin (LN) in UC and IP samples. sEVs were isolated by IP using an anti-CD63 antibody. (C) Western blot analysis of sEVs isolated by IP using anti-fibronectin antibody. Source data are available for this figure: SourceData FS3.