Integrin α2β1 in sEVs derived from PC3 cells is responsible for the binding of CD63-containing sEVs to collagen type I, and integrins α6β1 and α6β4 are responsible for the binding to laminin. (A, C, E, and G) Single-particle fluorescence images of sEV–CD63Halo7-SF650T on glass coated with fibronectin, collagen type I, and laminin before and after the KO of integrin β1 (A), integrin α2 (C), integrin α6 (E), and integrin β4 (G). (B, D, F, and H) The numbers of sEVs attached to glass coated with these ECM molecules before and after the KO of integrin β1 (B), integrin α2 (D), integrin α6 (F), and integrin β4 (H) (n = 16 images). Data are presented as the mean ± SE. n.s., nonsignificant difference; ***P < 0.001 according to Welch’s t test (two-sided).