Western blotting of integrin subunits in PC3 cells and sEVs and spreading assay of the cells that secreted sEVs in this study on all three ECMs (fibronectin, laminin, and collagen type I) coated on glass. (A) sEVs were isolated from the cell culture supernatant of intact PC3 cells or from cells in which an integrin subunit (β1, β4, α2, or α6) was knocked out via the CRISPR-Cas9 method. (B) The correlation map of integrin heterodimers and the ECM. (C) dSTORM images of the ECM (left-top: fibronectin, left-bottom: collagen type Ⅰ, and right: laminin) coated on glass. (D) Images of HeLa cells attached to glass coated with ECM components (fibronectin, collagen type Ⅰ, and laminin) or casein after 0, 30, or 60 min of incubation. (E) The time course of the area of five tumor cell lines on glass coated with ECM components (fibronectin, collagen type Ⅰ, and laminin) or casein (n = 8 cells). Data are presented as the mean ± SE. Source data are available for this figure: SourceData F1.