Figure S5.
Rab GTPases colocalize with LC3 or mitophagy receptors around damaged mitochondria under CCCP-treated conditions. (A) U2OS cells transiently expressing HA-GFP-Rab GTPases, were treated with DMSO or CCCP 10 µM for 4 h, stained with anti-TOMM20and anti-LC3, the colocalization of Rab GTPases, endogenous TOMM20 and LC3 was analyzed by confocal microscopy. Scale bars, 10 µm. The scale bars in the magnification boxes are 2 µm. (B) U2OS cells transiently expressing HA-GFP-Rab GTPases and mCherry-NDP52 (mCherry-OPTN), were treated with DMSO or CCCP for 4 h, stained with anti-TOMM20, the colocalization of Rab GTPases, mCherry-NDP52, mCherry-OPTN, or endogenous p62 and TOMM20 was analyzed by confocal microscopy. Scale bars, 10 µm. The scale bars in the magnification boxes are 2 µm. Molecular weight measurements are in kD.

Rab GTPases colocalize with LC3 or mitophagy receptors around damaged mitochondria under CCCP-treated conditions. (A) U2OS cells transiently expressing HA-GFP-Rab GTPases, were treated with DMSO or CCCP 10 µM for 4 h, stained with anti-TOMM20and anti-LC3, the colocalization of Rab GTPases, endogenous TOMM20 and LC3 was analyzed by confocal microscopy. Scale bars, 10 µm. The scale bars in the magnification boxes are 2 µm. (B) U2OS cells transiently expressing HA-GFP-Rab GTPases and mCherry-NDP52 (mCherry-OPTN), were treated with DMSO or CCCP for 4 h, stained with anti-TOMM20, the colocalization of Rab GTPases, mCherry-NDP52, mCherry-OPTN, or endogenous p62 and TOMM20 was analyzed by confocal microscopy. Scale bars, 10 µm. The scale bars in the magnification boxes are 2 µm. Molecular weight measurements are in kD.

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