The expression and subcellular distribution of TMBIM-2 in response to neuronal mitochondrial stress. (A) Quantification of tmbim-2 mRNA levels in young adult WT (yellow) and neuronal cox-5B knockdown (uthIs375[unc-119p::cox-5B HP; pRF(rol-6)]) (blue) animals. n ≥ 3 biologically independent samples. (B) Immunoblot of young adult TMBIM-2::GFP expression in WT and neuronal cox-5B knockdown animals. (C) Quantification of TMBIM-2::GFP protein levels as shown in B: WT (yellow) and neuronal cox-5B knockdown (blue) animals; n ≥ 3 biologically independent samples. (D) Representative confocal photomicrographs of day 1 adult animals with ythEx572[tmbim-2p::tmbim-2::gfp] in combination with a lipophilic plasma membrane dye DiD at the cell body. The imaging used Z-planes. Scale bar, 2 μm. Pearson’s correlation coefficient (r) for the ROI is 0.94. (E) Representative confocal photomicrographs of day 1 adult animals with TMBIM-2 in combination with an ADF synaptic vesicle marker ythEx536[srh-142p::tmbim-2::gfp+srh-142p::Scarlet::rab-3]. The imaging used Z-stacks. Scale bar, 5 μm. Pearson’s correlation coefficient (r) for the ROI is 0.90. (F) Representative Single Slice-SIM photomicrographs of ADF neuron overexpressing TMBIM-2 (ythIs102[srh-142p::tmbim-2::gfp]) animals with the presence or absence of neuronal cox-5B knockdown. The imaging used Z-planes. Scale bar, 10 μm. *P < 0.05 via unpaired two-tailed Student’s t test. Error bars, SEM. Source data are available for this figure: SourceData F2.