The expression and subcellular distribution of TMBIM-2 in response to neuronal mitochondrial stress. (A) Representative photomicrograph of dve-1 reporter in tmbim-2(yth57); neuronal Q40 overexpressing animals with or without TMBIM-2::GFP rescue. Scale bar, 250 μm. (B) Quantification of the number of intestinal nuclei puncta with GFP signal per worm as shown in A. n ≥ 12 worms. (C) The Dot plot shows the average expression of TMBIM-2 in kinds of C. elegans tissues. Expression values are from Roux et al. (2023). (D) Representative confocal photomicrographs of day 1 adult animals with ythIs62[tmbim-2p::tmbim-2::gfp] in combination with a pan-neuron marker rgef-1p::mcherry. Scale bar, 20 μm. Panels below show high-magnification views of boxed regions Zoom 1 (Head region), Zoom 2 (Spermatheca), and Zoom 3 (Tail region). The imaging used Z-planes. Scale bar, 5 μm. (E) Representative confocal photomicrographs of day 1 adult animals with ythIs62[tmbim-2p::tmbim-2::gfp] in combination with a neuronal mitochondria marker (forSi44[rgef-1p::tomm-20::mKate2::HA]). The imaging used Z-planes. Scale bar, 2 μm. Pearson’s correlation coefficient (r) for the ROI is −0.05. (F) Representative confocal photomicrographs of day 1 adult animals with ythIs62[tmbim-2p::tmbim-2::gfp] in combination with the synaptic active zone marker (ythEX774[xbx-1p::mcherry::syd-1]). The imaging used Z-planes. Scale bar, 2 μm. Pearson’s correlation coefficient (r) for the ROI is 0.63. (G) Representative confocal photomicrographs of day 1 adult animals with ADF neuron overexpressing TMBIM-2 (ythIs102[srh-142p::tmbim-2]) in combination with the trans-Golgi marker (TGN-38::mCherry). The imaging used Z-planes. Scale bar, 2 μm. (H) Representative confocal photomicrographs of day 1 adult animals with ADF neuron overexpressing TMBIM-2 (ythIs102[srh-142p::tmbim-2]) in combination with the medial/cis-Golgi marker (AMAN-2::mCherry). The imaging used Z-planes. Scale bar, 2 μm. ***P < 0.001 via unpaired two-tailed Student’s t test. Error bars, SEM.