Structure of TANGO2 and interaction simulation. (a) Mass distribution for TANGO2.Flag oligomers by mass photometry. (b) HepG2 cells cotransfected with TANGO2.Flag and TANGO2.mScarlet were immunoprecipitated (IP) with anti-flag resin followed by western blot analysis. The left and right panels show cell lysate input and IP, respectively. The top membrane shows immunoblotting against RFP. Flag and β-actin were used as IP and loading controls. (a–c) Cylinder (α-helices) and arrow (β-sheets) cartoon representation of the TANGO2 isoform 1 structure. The rainbow-colored chain indicates the beginning of the amino-terminal sequence (blue) to the carboxyl-terminal sequence (red) of TANGO2. The N° indicates the rotation degrees of the protein structure in the y axis. (d) Visualization of the cavitation (violet) from KVFinder in the predicted three-dimensional surface of the TANGO2 isoform 1 protein. (e) Position of the LIL and NRDE sequences in the TANGO2 structure and interaction simulation with one and two palmitate chains by AlphaFold 3.0. All structural representations of TANGO2 are based on AlphaFold prediction and visualized using UCSF ChimeraX. Source data are available for this figure: SourceData F4.