Figure 2. TANGO2 wild-type and mutant... | Rockefeller University Press

Figure 2.

$TANGO2 wild-type and mutant location. (a–c) HepG2 cells transfected with TANGO2.Iso1-mScarlet (a), or the mutants TANGO2.∆NRDE-mScarlet (b) and TANGO2.∆LIL-mScarlet (c) were incubated with the LD marker Bodipy Green and the mitochondrial marker MitoTracker Deep Red (cyan). (d and e) HepG2 cells expressing the mutant TANGO2.40aa-mScarlet (d) or TANGO2.40aa.∆LIL-mScarlet (e) were incubated with MitoTracker Green to detect the mitochondria and Hoechst-33342 (blue) to visualize DNA. (f and g) HepG2 cells expressing TANGO2.Iso1-mScarlet (f) or TANGO2.∆LIL-mScarlet (g) were co-expressed with the LD marker construct GPAT4.hairpin-NG (green) and incubated with Hoechst-33342 (blue) to visualize DNA. Pearson’s correlation (r) and Mander’s overlap (M1 and M2) coefficients were calculated with the coloc2 plugin in ImageJ software. Squares indicate the magnification area (inset). Scale bars = 10 µm. Images are representative of three independent experiments. (h) HepG2 cells were incubated in a low-glucose medium for different periods (hour) and mechanically lysed, and the cytoplasmic and whole-cell fractions were analyzed by western blot. β-Actin was used as a loading control. (i) Graph shows the relative expression of the TANGO2/β-actin ratio in the cytoplasmic and whole-cell fractions. Source data are available for this figure: SourceData F2.$

TANGO2 wild-type and mutant location. (a–c) HepG2 cells transfected with TANGO2.Iso1-mScarlet (a), or the mutants TANGO2.∆NRDE-mScarlet (b) and TANGO2.∆LIL-mScarlet (c) were incubated with the LD marker Bodipy Green and the mitochondrial marker MitoTracker Deep Red (cyan). (d and e) HepG2 cells expressing the mutant TANGO2.40aa-mScarlet (d) or TANGO2.40aa.∆LIL-mScarlet (e) were incubated with MitoTracker Green to detect the mitochondria and Hoechst-33342 (blue) to visualize DNA. (f and g) HepG2 cells expressing TANGO2.Iso1-mScarlet (f) or TANGO2.∆LIL-mScarlet (g) were co-expressed with the LD marker construct GPAT4.hairpin-NG (green) and incubated with Hoechst-33342 (blue) to visualize DNA. Pearson’s correlation (r) and Mander’s overlap (M1 and M2) coefficients were calculated with the coloc2 plugin in ImageJ software. Squares indicate the magnification area (inset). Scale bars = 10 µm. Images are representative of three independent experiments. (h) HepG2 cells were incubated in a low-glucose medium for different periods (hour) and mechanically lysed, and the cytoplasmic and whole-cell fractions were analyzed by western blot. β-Actin was used as a loading control. (i) Graph shows the relative expression of the TANGO2/β-actin ratio in the cytoplasmic and whole-cell fractions. Source data are available for this figure: SourceData F2.

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