Figure 1. Localization of TANGO2... | Rockefeller University Press

Figure 1.

$Localization of TANGO2 isoforms 1, 2, and 5. (a) HepG2 cells expressing TANGO2.Iso1-mScarlet (magenta) were incubated with Hoechst-33342 (blue) to detect DNA and MitoTracker Green to visualize the mitochondria. Pearson’s correlation (r) and Mander’s overlap (M1 and M2) coefficients were calculated with the coloc2 plugin in ImageJ software. (b) Cells cotransfected with TANGO2.Iso1-mScarlet (magenta) and Tom20.GFP (green) were incubated with Hoechst-33342 (blue) to detect DNA. (c) HepG2 cells were processed to obtain mitochondria-enriched fractions. The mitochondrial fractions were treated (+) with 50 µg/ml proteinase K for outer membrane protein cleavage and 1% Triton X-100 for total membrane disruption. Samples were resolved by SDS-PAGE and incubated with the antibodies Tom20, sited in the outer mitochondrial membrane, and ATP5A, sited in the inner mitochondrial membrane, and TANGO2. OMM, outer mitochondrial membrane; IMM, inner mitochondrial membrane. (d and e) HepG2 cells expressing TANGO2.Iso2-mScarlet (d) or TANGO2.Iso5-mScarlet (e) were incubated with MitoTracker Green to detect the mitochondria and Hoechst-33342 (blue) to label DNA. Pearson’s correlation (r) and Mander’s overlap (M1 and M2) coefficients were calculated with the coloc2 plugin in ImageJ software. Squares indicate the magnification area (inset). Scale bars = 10 µm. The white color indicates colocalization between magenta and green channels. Images are representative of three independent experiments. Source data are available for this figure: SourceData F1.$

Localization of TANGO2 isoforms 1, 2, and 5. (a) HepG2 cells expressing TANGO2.Iso1-mScarlet (magenta) were incubated with Hoechst-33342 (blue) to detect DNA and MitoTracker Green to visualize the mitochondria. Pearson’s correlation (r) and Mander’s overlap (M1 and M2) coefficients were calculated with the coloc2 plugin in ImageJ software. (b) Cells cotransfected with TANGO2.Iso1-mScarlet (magenta) and Tom20.GFP (green) were incubated with Hoechst-33342 (blue) to detect DNA. (c) HepG2 cells were processed to obtain mitochondria-enriched fractions. The mitochondrial fractions were treated (+) with 50 µg/ml proteinase K for outer membrane protein cleavage and 1% Triton X-100 for total membrane disruption. Samples were resolved by SDS-PAGE and incubated with the antibodies Tom20, sited in the outer mitochondrial membrane, and ATP5A, sited in the inner mitochondrial membrane, and TANGO2. OMM, outer mitochondrial membrane; IMM, inner mitochondrial membrane. (d and e) HepG2 cells expressing TANGO2.Iso2-mScarlet (d) or TANGO2.Iso5-mScarlet (e) were incubated with MitoTracker Green to detect the mitochondria and Hoechst-33342 (blue) to label DNA. Pearson’s correlation (r) and Mander’s overlap (M1 and M2) coefficients were calculated with the coloc2 plugin in ImageJ software. Squares indicate the magnification area (inset). Scale bars = 10 µm. The white color indicates colocalization between magenta and green channels. Images are representative of three independent experiments. Source data are available for this figure: SourceData F1.

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