Effect of Stim1/2 ablation on neutrophil extravasation during inflammation. (A) Fluorescence micrograph of cremaster tissue from WT and Stim1/2^KO mice, injected i.p. with 2.5 µg/ml TNF for 2 h prior to sacrifice and surgical extraction of the cremaster muscles. Granulocytes are stained white with GR-1, blood vessels blue with CD31 antibodies. (B) Quantification of interstitial GR-1⁺ cells within a distance of 75 µm from postcapillary venules in tissue sections from WT and Stim1/2^KO mice. n = 57/60 vessels from four mice pairs, exact two-tailed Mann–Whitney U test. Small and large symbols show data from individual vessels and mouse average, lines show median values by vessel.