Figure S6. The effect of Stim1/2... | Rockefeller University Press

Figure S6.

The effect of Stim1/2 ablation on IP3R mRNA levels and on neutrophil actin dynamics, related toFig. 6.(A) Real-time qPCR of neutrophils purified from WT and Stim1/2-deficient with primers designed against the indicated targets. n = 3 mice pairs, paired t test. (B) Quantification of adhesive membranes length (left) and actin coating thickness (right) in the electron micrographs of Fig. 7 A. n = 26/19 cells from two mice pairs, exact two-tailed Mann–Whitney U test. (C) Sir-actin kymographs of WT and Stim1/2-deficient neutrophils during spreading. Dotted lines on micrographs at left indicate the kymograph axis. Scale bar: 10 µm. (D) Representative micrographs of spread WT and Stim1/2-deficient neutrophils stained with phalloidin (left) and quantification of phalloidin staining intensity (right, n = 44/45 cells in four experiments from two mice pairs, exact two-tailed Mann–Whitney U test).

The effect of Stim1/2 ablation on IP ₃ R mRNA levels and on neutrophil actin dynamics, related to Fig. 6 . (A) Real-time qPCR of neutrophils purified from WT and Stim1/2-deficient with primers designed against the indicated targets. n = 3 mice pairs, paired t test. (B) Quantification of adhesive membranes length (left) and actin coating thickness (right) in the electron micrographs of Fig. 7 A. n = 26/19 cells from two mice pairs, exact two-tailed Mann–Whitney U test. (C) Sir-actin kymographs of WT and Stim1/2-deficient neutrophils during spreading. Dotted lines on micrographs at left indicate the kymograph axis. Scale bar: 10 µm. (D) Representative micrographs of spread WT and Stim1/2-deficient neutrophils stained with phalloidin (left) and quantification of phalloidin staining intensity (right, n = 44/45 cells in four experiments from two mice pairs, exact two-tailed Mann–Whitney U test).