Figure S3. Effect of Stim1/2 ablation on... | Rockefeller University Press

Figure S3.

$Effect of Stim1/2 ablation on the spreading of neutrophils. (A) Effective and cumulative distance covered by the centroids of WT and Stim1/2−/− neutrophils tracked in Fig. 4 A. Panels at right show the cross-correlation of these parameters. Bottom graphs show the centroid location and position relative to the initial landing spot and centroids probability distributions. (B) DIC micrographs (left) and fraction of spread WT and Stim1/2−/− neutrophils treated or not with U72133 following ∼20-min plating at 37°C (right, n = 37/20 and 39/9 recordings from three WT/Stim1/2−/− mice pairs, two-tailed Welch’s t test). (C) Change in the size of TIRF footprints of WT neutrophils treated or not with U72133 (n = 25/25 graphed and 34/19 analyzed cells from three WT/Stim1/2−/− mice pairs, two-tailed Welch’s t test).$

Effect of Stim1/2 ablation on the spreading of neutrophils. (A) Effective and cumulative distance covered by the centroids of WT and Stim1/2^−/− neutrophils tracked in Fig. 4 A. Panels at right show the cross-correlation of these parameters. Bottom graphs show the centroid location and position relative to the initial landing spot and centroids probability distributions. (B) DIC micrographs (left) and fraction of spread WT and Stim1/2^−/− neutrophils treated or not with U72133 following ∼20-min plating at 37°C (right, n = 37/20 and 39/9 recordings from three WT/Stim1/2^−/− mice pairs, two-tailed Welch’s t test). (C) Change in the size of TIRF footprints of WT neutrophils treated or not with U72133 (n = 25/25 graphed and 34/19 analyzed cells from three WT/Stim1/2^−/− mice pairs, two-tailed Welch’s t test).