Figure S2. Effect of pharmacological... | Rockefeller University Press

Figure S2.

Effect of pharmacological inhibitors on neutrophil Ca2+responses. (A) Salsa6f recordings (left), Ca2+ transient frequency (middle), and integrated Ca2+ responses (right) of adherent neutrophils exposed to CM4620 (10 μM), Gd3+ (10 μM), or to the PKC activator PMA (1 μM). n = 27/42/35 cells in 4/4/3 recordings from two mice, Student’s paired two-tailed t test. (B) Entire recordings of Fig. 3 A showing the subsequent sequential addition of fMIVIL (10 nM) and Tg (1 μM) to cells treated with DMSO, GSK-7975a, and U73122. (C) Effect of the indicated inhibitors on Ca2+ responses evoked by fMIVIL and Tg. n = 92/40/35/45 cells for fMIVIL with DMSO/DPI/PMA/U73 and 68/44/31 cells for Tg with DMSO/GSK/EGTA from two to eight mice. Two-tailed Welch’s t test. (D) Effect of DMSO, GSK7975a, and CM4620 on the Ca2+ responses evoked by Ca2+ readmission to cells treated with Tg in Ca2+-free medium. n = 125/126/116 cells in 4/4/4 recordings from two mice. Ordinary one-way ANOVA with Šídák multiple comparison test. Gd3+: gadolinium; fMIVIL: N-formyl-Met-Ile-Val-Ile-Leu; AUC: area under the curve.

Effect of pharmacological inhibitors on neutrophil Ca ²⁺ responses. (A) Salsa6f recordings (left), Ca²⁺ transient frequency (middle), and integrated Ca²⁺ responses (right) of adherent neutrophils exposed to CM4620 (10 μM), Gd³⁺ (10 μM), or to the PKC activator PMA (1 μM). n = 27/42/35 cells in 4/4/3 recordings from two mice, Student’s paired two-tailed t test. (B) Entire recordings of Fig. 3 A showing the subsequent sequential addition of fMIVIL (10 nM) and Tg (1 μM) to cells treated with DMSO, GSK-7975a, and U73122. (C) Effect of the indicated inhibitors on Ca²⁺ responses evoked by fMIVIL and Tg. n = 92/40/35/45 cells for fMIVIL with DMSO/DPI/PMA/U73 and 68/44/31 cells for Tg with DMSO/GSK/EGTA from two to eight mice. Two-tailed Welch’s t test. (D) Effect of DMSO, GSK7975a, and CM4620 on the Ca²⁺ responses evoked by Ca²⁺ readmission to cells treated with Tg in Ca²⁺-free medium. n = 125/126/116 cells in 4/4/4 recordings from two mice. Ordinary one-way ANOVA with Šídák multiple comparison test. Gd^3+: gadolinium; fMIVIL: N-formyl-Met-Ile-Val-Ile-Leu; AUC: area under the curve.