Figure 3.
![Effect of Orai1 and PLC inhibition on Ca2+signals during neutrophils spreading. (A) Salsa6f recordings (left), Ca2+ transient frequency (middle), and integrated Ca2+ responses (right) of flushed neutrophils adhered to PLL and exposed to DMSO (0.1%), the Orai1 inhibitor GSK-7975a (10 μM), or the PLC inhibitor U73122 (1 μM) in 2 mM [Ca2+]ext. n = 436/83/56 cells in 10/4/4 recordings from four to eight mice, Student’s paired two-tailed t test. (B) Time-course micrographs of bottom recordings in A. See Video 5.](https://cdn.rupress.org/rup/content_public/journal/jcb/224/5/10.1083_jcb.202406053/3/jcb_202406053_fig3.png?Expires=1773504358&Signature=OLk8WUP4iaP2UvwUyQGiLtJG~4gr-5qAFUVrpJcdGTHsAAOnErl~KCmyZtbRtSaXLR3hvdEMlbx785NQd7uzpR1bNUhAvXQtaKtZsjJE2IdxHqorilbcHSoBHBcX3F4ghHybJd8YPFs8m4I~L2T9JwLdAqiTPD4BgOg7E7SXvfaPXOFPra2bgwsHuPg-ySi2h9zjM~ni38-p05~WB2JYibKSt33Dwwkz0PScn4rxRT-7zoSYFf06ZpaTX4ehkcmYN-F3r1VYP1IXSYyk9pHqfyyyqPPTgyI1GHooTMMlPxnLHPHEi7HMwlQLL2Ilxeqjs8Iw~p0XZ-YO9DxuJjaxYA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of Orai1 and PLC inhibition on Ca 2+ signals during neutrophils spreading. (A) Salsa6f recordings (left), Ca2+ transient frequency (middle), and integrated Ca2+ responses (right) of flushed neutrophils adhered to PLL and exposed to DMSO (0.1%), the Orai1 inhibitor GSK-7975a (10 μM), or the PLC inhibitor U73122 (1 μM) in 2 mM [Ca2+]ext. n = 436/83/56 cells in 10/4/4 recordings from four to eight mice, Student’s paired two-tailed t test. (B) Time-course micrographs of bottom recordings in A. See Video 5.
