Figure 2. Stim1/2 ablation prevents... | Rockefeller University Press

Figure 2.

Stim1/2 ablation prevents repetitive Ca2+signals during neutrophils spreading. (A) Fluo-8 recordings and quantification of Ca2+ responses evoked by Tg and fMIVIL in WT and Stim1/2KO-purified neutrophils suspended in physiological saline. n = 6/5 recordings from three pairs of mice, Student’s unpaired one-tailed t test. (B) Single-cell Salsa6f recordings and quantification of Ca2+ responses evoked by Tg and fMIVIL in flushed WT and Stim1/2KO neutrophils adhered to PLL. n = 6/5 recordings from three pairs of mice, mean ± SEM, Student’s unpaired one-tailed t test. (C) Salsa6f micrographs and recordings of flushed cells adhered to PLL coatings (left, see Video 3 and Video 4) and incidence of Ca2+ transients and integrated Ca2+ responses (right, n = 25/19 recordings from three mice pairs, two-tailed Welch’s t test). fMIVIL; N-formyl-Met-Ile-Val-Ile-Leu.

Stim1/2 ablation prevents repetitive Ca ²⁺ signals during neutrophils spreading. (A) Fluo-8 recordings and quantification of Ca²⁺ responses evoked by Tg and fMIVIL in WT and Stim1/2^KO-purified neutrophils suspended in physiological saline. n = 6/5 recordings from three pairs of mice, Student’s unpaired one-tailed t test. (B) Single-cell Salsa6f recordings and quantification of Ca²⁺ responses evoked by Tg and fMIVIL in flushed WT and Stim1/2^KO neutrophils adhered to PLL. n = 6/5 recordings from three pairs of mice, mean ± SEM, Student’s unpaired one-tailed t test. (C) Salsa6f micrographs and recordings of flushed cells adhered to PLL coatings (left, see Video 3 and Video 4) and incidence of Ca²⁺ transients and integrated Ca²⁺ responses (right, n = 25/19 recordings from three mice pairs, two-tailed Welch’s t test). fMIVIL; N-formyl-Met-Ile-Val-Ile-Leu.