s nx10 is highly expressed in the brain of zebrafish larvae and its depletion elevates oxidative stress. (A) Dorsal view of spatial expression pattern of snx10a and snx10b at 2, 4, and 5 dpf as demonstrated by WM-ISH using an internal antisense (AS) probe. Scale bar = 200 μm. Images are representative of three experiments. Control 2 dpf larvae hybridized to a sense probe (S). (B) Illustration of sgRNA-binding regions on snx10a and snx10b gene, respectively. (C) Temporal expression levels of bnip3 and bnip3l transcripts in DMSO- and DMOG-treated WT zebrafish larvae at 3 dpf. The graph shows the fold change in transcript levels relative to β-actin and normalized to DMSO 2^−ΔΔCt levels. Error bars indicate mean ± SEM. Data are collected from three individual experiments. Significance was determined by two-way ANOVA test to compare all groups with the two variables. (D) Temporal expression levels of cox-iv and samm50 transcripts in control and snx10ab_DKO zebrafish larvae treated with or without 100 µm DMOG at 3 dpf. The graph shows the fold change in transcript levels relative to β-actin and normalized to DMSO 2^−ΔΔCt levels. Error bars indicate mean ± SEM. Data are collected from three individual experiments. Significance was determined by one-way ANOVA test to compare all groups with the individual variable. Data distribution was assumed to be normal but was not formally tested. * = P < 0.05, ** = P < 0.01, *** = P < 0.001, and **** = P < 0.0001; nonsignificant differences are not depicted. (E) Representative dot plots showing the region selected for FACS analysis from control and snx10ab DKO zebrafish larvae at 3 dpf using MitoSOX reagent. H₂O₂ was added to the water for 1 h as a positive control. (F) Representative FACS plot showing oxidative stress in control and snx10ab DKO zebrafish larvae at 3 dpf using the MitoSOX reagent. H₂O₂ added in water served as positive control.