Figure 6.
SNX10 is a negative modulator of COX-IV turnover. (A) U2OS cells were reverse transfected with the indicated siRNA (20 nM) for 72 h, then treated or not with DFP (1 µM) for 24 h and with BafA1 (50 nM) the last 16 h, followed by western blotting for the indicated proteins. (B–D) Quantification of the data in A from n = 5, 3, and 6 independent experiments. Bars show mean values of the protein levels normalized to actin relative to control conditions (siCtrl control) ± SEM. Significance is assessed by two-way ANOVA followed by Tukey’s post hoc test. Data distribution was assumed to be normal. (E) U2OS cells with stable expression of mScarlet-RAB5 were reverse transfected with the indicated siRNA (20 nM) for 72 h, then treated or not with DFP for 24 h. The cells were fixed and stained with anti–COX-IV antibody before image acquisition. Scale bar: 10 µm. Insets: 3.69 × 3.69 µm. (F) Quantification of COX-IV intensity from E represented as z-score from two independent experiments (>250 cells per experiment). The statistical significance between the control and the other conditions was calculated with ordinary one-way ANOVA followed by Tukey’s multiple comparison test. Data distribution was assumed to be normal but was not formally tested. * = P < 0.05, ** = P < 0.01, *** = P < 0.001, and **** = P < 0.0001; nonsignificant differences are not depicted. BafA1; bafilomycin A1. Source data are available for this figure: SourceData F6.

SNX10 is a negative modulator of COX-IV turnover. (A) U2OS cells were reverse transfected with the indicated siRNA (20 nM) for 72 h, then treated or not with DFP (1 µM) for 24 h and with BafA1 (50 nM) the last 16 h, followed by western blotting for the indicated proteins. (B–D) Quantification of the data in A from n = 5, 3, and 6 independent experiments. Bars show mean values of the protein levels normalized to actin relative to control conditions (siCtrl control) ± SEM. Significance is assessed by two-way ANOVA followed by Tukey’s post hoc test. Data distribution was assumed to be normal. (E) U2OS cells with stable expression of mScarlet-RAB5 were reverse transfected with the indicated siRNA (20 nM) for 72 h, then treated or not with DFP for 24 h. The cells were fixed and stained with anti–COX-IV antibody before image acquisition. Scale bar: 10 µm. Insets: 3.69 × 3.69 µm. (F) Quantification of COX-IV intensity from E represented as z-score from two independent experiments (>250 cells per experiment). The statistical significance between the control and the other conditions was calculated with ordinary one-way ANOVA followed by Tukey’s multiple comparison test. Data distribution was assumed to be normal but was not formally tested. * = P < 0.05, ** = P < 0.01, *** = P < 0.001, and **** = P < 0.0001; nonsignificant differences are not depicted. BafA1; bafilomycin A1. Source data are available for this figure: SourceData F6.

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