Figure 5.
SNX10 vesicles contain mitochondrial proteins and LC3B. (A) U2OS cells with stable inducible expression of SNX10-EGFP were pre-treated with doxycycline for 16 h before the addition of DFP (1 µM) for 24 h. The cells were fixed and stained with antibodies against mitochondrial proteins. Scale bars: 10 µm. Insets: 8.57 × 8.57 µm. (B and D) U2OS cells with inducible expression of SNX10-EGFP were treated with DFP (1 µM) or DMOG (1 µM) for 24 h and stained with antibodies anti–COX-IV and anti-LC3B in B or anti-LAMP1 in D, prior to acquisition with a Nikon CREST X-Light V3 spinning disk microscope using a 60× oil objective (NA 1.42). Scale bar: 10 µm. Insets: 4.41 × 4.41 µm (B), 5.52 × 5.52 µm (D). (C–E) Pixel intensity plots for line in control, DFP, and DMOG insets, respectively for (B and D).

SNX10 vesicles contain mitochondrial proteins and LC3B. (A) U2OS cells with stable inducible expression of SNX10-EGFP were pre-treated with doxycycline for 16 h before the addition of DFP (1 µM) for 24 h. The cells were fixed and stained with antibodies against mitochondrial proteins. Scale bars: 10 µm. Insets: 8.57 × 8.57 µm. (B and D) U2OS cells with inducible expression of SNX10-EGFP were treated with DFP (1 µM) or DMOG (1 µM) for 24 h and stained with antibodies anti–COX-IV and anti-LC3B in B or anti-LAMP1 in D, prior to acquisition with a Nikon CREST X-Light V3 spinning disk microscope using a 60× oil objective (NA 1.42). Scale bar: 10 µm. Insets: 4.41 × 4.41 µm (B), 5.52 × 5.52 µm (D). (C–E) Pixel intensity plots for line in control, DFP, and DMOG insets, respectively for (B and D).

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