Figure 4.
SNX10 structures containing mitochondria mature into late endosomes. (A and C) U2OS cells with inducible expression of SNX10-EGFP were treated with DFP (1 µM) or DMOG (1 µM) for 24 h, stained with MitoTracker Deep Red FM (100 nM) for 30 min, followed by immunofluorescence with antibodies anti-LC3B and anti-EEA1 (A) or anti-CD63 (C), prior to acquisition with a Nikon CREST X-Light V3 spinning disk microscope using a 60× oil objective (NA 1.42). Scale bar: 10 µm. Insets: 5.52 × 5.52 µm (A), 6.62 × 6.62 µm (C). (B–D) Pixel intensity plots for line in control, DFP, and DMOG insets, respectively for A and C.

SNX10 structures containing mitochondria mature into late endosomes. (A and C) U2OS cells with inducible expression of SNX10-EGFP were treated with DFP (1 µM) or DMOG (1 µM) for 24 h, stained with MitoTracker Deep Red FM (100 nM) for 30 min, followed by immunofluorescence with antibodies anti-LC3B and anti-EEA1 (A) or anti-CD63 (C), prior to acquisition with a Nikon CREST X-Light V3 spinning disk microscope using a 60× oil objective (NA 1.42). Scale bar: 10 µm. Insets: 5.52 × 5.52 µm (A), 6.62 × 6.62 µm (C). (B–D) Pixel intensity plots for line in control, DFP, and DMOG insets, respectively for A and C.

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