SNX10 localizes to endocytic structures. (A) Fluorescence imaging of U2OS cell lines stably expressing doxycycline-inducible SNX10-EGFP WT or the indicated ARO-linked mutants was acquired at 20× magnification using a Zeiss Axio Observer widefield microscope (Zen Blue 2.3; Zeiss). Corresponding brightfield images are displayed below the fluorescence images. Pink arrows correspond to observed vacuoles. Scale bar = 20 µm. (C) U2OS cells stably expressing SNX10-EGFP were treated with LysoTracker Red prior to fixation. Scale bars: 10 μm. Insets: 8.40 × 8.40 µm. (B) U2OS cells with stable inducible expression of SNX10-EGFP were infected with lentiviral particles to express mScarlet-RAB4/RAB5/RAB6/RAB7/RAB9/RAB11/RAB43. Nuclei were stained with Hoechst. Scale bars: 10 μm. Insets: 10.92 × 10.92 µm. (D) U2OS cells with stable inducible expression of SNX10-EGFP were fixed and stained against endogenous clathrin. Nuclei were stained with Hoechst. Scale bar: 10 μm. Insets: 9.48 × 9.48 µm.