Figure 7.
SCLIM analysis of CUPS-modified TGN contacts. (A) Grh1-2xmCherry (magenta) and GFP-Tlg2 (green) cells cultured in the starvation condition for 1.5 h. 3D time-lapse images (10-s intervals) are indicated. Light blue arrows show separated membrane structures labeled with GFP-Tlg2. Yellow arrows indicate where the membrane structures have been cut. White arrows indicate where Grh1 contacts with a Tlg2 protrusive membrane. Scale bar = 0.5 µm. (B) Grh1-2xCherry (magenta) and Drs2-3xGFP (green) cells cultured in the starvation condition for 1.5 h. 3D time-lapse images (20-s intervals) are indicated. Light blue arrows show separated membrane structures labeled with Drs2-3xGFP. Yellow arrows indicate where the membrane structures have been cut. White arrows indicate where Grh1 contacts with a Drs2 protrusive membrane. Scale bar = 0.5 µm.

SCLIM analysis of CUPS-modified TGN contacts. (A) Grh1-2xmCherry (magenta) and GFP-Tlg2 (green) cells cultured in the starvation condition for 1.5 h. 3D time-lapse images (10-s intervals) are indicated. Light blue arrows show separated membrane structures labeled with GFP-Tlg2. Yellow arrows indicate where the membrane structures have been cut. White arrows indicate where Grh1 contacts with a Tlg2 protrusive membrane. Scale bar = 0.5 µm. (B) Grh1-2xCherry (magenta) and Drs2-3xGFP (green) cells cultured in the starvation condition for 1.5 h. 3D time-lapse images (20-s intervals) are indicated. Light blue arrows show separated membrane structures labeled with Drs2-3xGFP. Yellow arrows indicate where the membrane structures have been cut. White arrows indicate where Grh1 contacts with a Drs2 protrusive membrane. Scale bar = 0.5 µm.

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