Figure 5.
SCLIM reveals the dynamic structure of CUPS. (A) Grh1-2xGFP cells were incubated in normal growth conditions or 2% potassium acetate for 3 h and visualized by SCLIM. In growth, Grh1 labeled many small and mobile structures (early Golgi membranes and ER exit sites). In starvation, Grh1 labeled fewer, larger, and less mobile membrane structures (CUPS) (arrowheads). Grid = 1.52 μm. (B and C) Line-scan analysis in 3D of multiple CUPS structures revealed three forms: spherical (3/14), complex curved (8/14), or cup-shaped (3/14). (D) Visualization of CUPS over time showed stable, mature CUPS are still dynamic, able to change morphology between the different forms. Region 1 = moving structures; Region 2 = nonmoving structure. The time-lapse images of Region 2 at 25, 50, and 75 frames are shown in XYZ images. Scale bar = 1 μm.

SCLIM reveals the dynamic structure of CUPS. (A) Grh1-2xGFP cells were incubated in normal growth conditions or 2% potassium acetate for 3 h and visualized by SCLIM. In growth, Grh1 labeled many small and mobile structures (early Golgi membranes and ER exit sites). In starvation, Grh1 labeled fewer, larger, and less mobile membrane structures (CUPS) (arrowheads). Grid = 1.52 μm. (B and C) Line-scan analysis in 3D of multiple CUPS structures revealed three forms: spherical (3/14), complex curved (8/14), or cup-shaped (3/14). (D) Visualization of CUPS over time showed stable, mature CUPS are still dynamic, able to change morphology between the different forms. Region 1 = moving structures; Region 2 = nonmoving structure. The time-lapse images of Region 2 at 25, 50, and 75 frames are shown in XYZ images. Scale bar = 1 μm.

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