CUPS contain a pool of the v-SNARE Snc2 and the t-SNARE Tlg2. (A and B) Cells genomically expressing Grh1-2xCherry with GFP-Snc1, GFP-Snc2, or GFP-Tlg2 were visualized by confocal spinning-disk microscopy in growth conditions and (B) throughout the time course of culture in 2% potassium acetate. Short movies were acquired at 10-s intervals to assess the frequency and duration of colocalization. Scale bar = 2 μm.