Figure 4.
EHBP-1 contributes to the sequestration of RAB-10-associated endosomes onto recycling endosomes. (A and A′) Confocal images showing colocalization between mNeonGreen-3xFlag-RAB-10KI and Mito-mCherry-RAB-10. (B and B′) Confocal images showing colocalization between VIT-2-mNeonGreen-3xFlagKI and mitochondria in different genetic backgrounds. (C and C′) Confocal images showing colocalization between GFP-RAB-10 and Tubby-PHR332H-mCherry. (D) Cartoon picture showing the working model of RFPnb-mediated protein translocation. (E and E′) Confocal images showing colocalization between Tubby-PHR332H-GFP and DHS-3-mCherry. The statistical analysis of colocalization was calculated as Pearson’s correlation coefficient and is shown as the mean ± SD (n = 18 cells from six animals of each genotype; one-way ANOVA test with Dunn’s multiple comparison in B′, C′, and E′; two-tailed Mann–Whitney test in A′). Refer to the image caption for details.

EHBP-1 contributes to the sequestration of RAB-10-associated endosomes onto recycling endosomes. (A and A′) Confocal images showing colocalization between mNeonGreen-3xFlag-RAB-10KI and Mito-mCherry-RAB-10. (B and B′) Confocal images showing colocalization between VIT-2-mNeonGreen-3xFlagKI and mitochondria in different genetic backgrounds. (C and C′) Confocal images showing colocalization between GFP-RAB-10 and Tubby-PHR332H-mCherry. (D) Cartoon picture showing the working model of RFPnb-mediated protein translocation. (E and E′) Confocal images showing colocalization between Tubby-PHR332H-GFP and DHS-3-mCherry. The statistical analysis of colocalization was calculated as Pearson’s correlation coefficient and is shown as the mean ± SD (n = 18 cells from six animals of each genotype; one-way ANOVA test with Dunn’s multiple comparison in B′, C′, and E′; two-tailed Mann–Whitney test in A′).

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