Figure 2.
The recycling regulators RME-1 and LET-413 function in the basolateral exocytic trafficking pathway, operating downstream of RAB-10 and EHBP-1. (A–E) Confocal images showing colocalization between VIT-2 and CED-10, LET-413, RME-1, or hTAC. Statistical analysis of colocalization was calculated as Pearson’s correlation coefficient and is shown as the mean ± SD (n = 18 cells from six animals of each genotype). (F) The intensity of VIT-2-mNeonGreen-3xFlagKI-labeled structures in the intestinal top, middle, or embryos was measured after RNAi-mediated knockdown of various endocytic recycling regulators. The data are shown as box-and-whisker plots with 10th–90th percentile (n = 24 cells from eight animals of each genotype; dots, outliers; boundaries, quartiles; one-way ANOVA test with Dunn’s multiple comparison). (G and G′) Confocal images showing the distribution of VIT-2-mNeonGreen-3xFlagKI in the C. elegans intestine, oocytes, and embryos. White dashed lines indicate the outlines of the intestine, oocytes, or embryos. The data are shown as box-and-whisker plots with 10th–90th percentile (n = 24 cells from eight animals of each genotype; dots, outliers; boundaries, quartiles; two-way ANOVA with Bonferroni post-test). (H and H′) Confocal images showing colocalization between VIT-2-mNeonGreen-3xFlagKI and Tubby-PHR332H-mCherry in different genetic backgrounds. Statistical analysis of colocalization was calculated as Pearson’s correlation coefficient and is shown as the mean ± SD (n = 18 cells from six animals of each genotype, one-way ANOVA test with Dunn’s multiple comparison). Refer to the image caption for details.

The recycling regulators RME-1 and LET-413 function in the basolateral exocytic trafficking pathway, operating downstream of RAB-10 and EHBP-1. (A–E) Confocal images showing colocalization between VIT-2 and CED-10, LET-413, RME-1, or hTAC. Statistical analysis of colocalization was calculated as Pearson’s correlation coefficient and is shown as the mean ± SD (n = 18 cells from six animals of each genotype). (F) The intensity of VIT-2-mNeonGreen-3xFlagKI-labeled structures in the intestinal top, middle, or embryos was measured after RNAi-mediated knockdown of various endocytic recycling regulators. The data are shown as box-and-whisker plots with 10th–90th percentile (n = 24 cells from eight animals of each genotype; dots, outliers; boundaries, quartiles; one-way ANOVA test with Dunn’s multiple comparison). (G and G′) Confocal images showing the distribution of VIT-2-mNeonGreen-3xFlagKI in the C. elegans intestine, oocytes, and embryos. White dashed lines indicate the outlines of the intestine, oocytes, or embryos. The data are shown as box-and-whisker plots with 10th–90th percentile (n = 24 cells from eight animals of each genotype; dots, outliers; boundaries, quartiles; two-way ANOVA with Bonferroni post-test). (H and H′) Confocal images showing colocalization between VIT-2-mNeonGreen-3xFlagKI and Tubby-PHR332H-mCherry in different genetic backgrounds. Statistical analysis of colocalization was calculated as Pearson’s correlation coefficient and is shown as the mean ± SD (n = 18 cells from six animals of each genotype, one-way ANOVA test with Dunn’s multiple comparison).

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