Figure 1.
RAB-10 and EHBP-1 participate in basolateral exocytosis in C. elegans intestinal epithelia. (A) A diagram of C. elegans intestine, showing the secretion pathway of yolk. (B and B′) Confocal images showing the distribution of VIT-2-mNeonGreen-3xFlagKI in the C. elegans intestine, oocytes, and embryos. “Top” shows the basal membrane, while “Middle” shows the apical membrane, cytosol, and lumen of the intestinal cells. White dashed lines indicate the outlines of the intestine, oocytes, or embryos. Data are shown as box-and-whisker plots with 10th–90th percentile (n = 24 cells from eight animals of each genotype; dots, outliers; boundaries, quartiles; one-way ANOVA test with Dunn’s multiple comparison). (C and C′) Western blot analysis of apoB protein levels in HepG2 cells. Band intensity was measured using the “Plot Lanes” function in ImageJ from three independent experiments. Error bars represent 95% CIs (one-way ANOVA test with Dunn’s multiple comparison). (D–D″) Transmission electron microscope (TEM) images of intestines and one-cell or two-cell embryos. The yellow arrowhead indicates a yolk granule (mv: microvilli; L: lysosome; M: mitochondria; ER: endoplasmic reticulum; LD: lipid droplet). Quantification of yolk number and diameter in C. elegans intestine, data are shown as box-and-whisker plots with 10th–90th percentile (n = 6 animals; dots, outliers; boundaries, quartiles; one-way ANOVA test with Dunn’s multiple comparison). (E and E′) Western blot analysis of VIT-2 protein levels in C. elegans early embryos using an anti-Flag antibody. The band intensity was measured using the “Plot Lanes” function in ImageJ from three independent experiments. The error bars represent 95% CIs (one-way ANOVA test with Dunn’s multiple comparison). (F and F′) Confocal images showing the subcellular localization of SLCF-1-GFP. Statistical analysis was conducted as in B. Source data are available for this figure: SourceData F1. Refer to the image caption for details.

RAB-10 and EHBP-1 participate in basolateral exocytosis in C. elegans intestinal epithelia. (A) A diagram of C. elegans intestine, showing the secretion pathway of yolk. (B and B′) Confocal images showing the distribution of VIT-2-mNeonGreen-3xFlagKI in the C. elegans intestine, oocytes, and embryos. “Top” shows the basal membrane, while “Middle” shows the apical membrane, cytosol, and lumen of the intestinal cells. White dashed lines indicate the outlines of the intestine, oocytes, or embryos. Data are shown as box-and-whisker plots with 10th–90th percentile (n = 24 cells from eight animals of each genotype; dots, outliers; boundaries, quartiles; one-way ANOVA test with Dunn’s multiple comparison). (C and C′) Western blot analysis of apoB protein levels in HepG2 cells. Band intensity was measured using the “Plot Lanes” function in ImageJ from three independent experiments. Error bars represent 95% CIs (one-way ANOVA test with Dunn’s multiple comparison). (D–D″) Transmission electron microscope (TEM) images of intestines and one-cell or two-cell embryos. The yellow arrowhead indicates a yolk granule (mv: microvilli; L: lysosome; M: mitochondria; ER: endoplasmic reticulum; LD: lipid droplet). Quantification of yolk number and diameter in C. elegans intestine, data are shown as box-and-whisker plots with 10th–90th percentile (n = 6 animals; dots, outliers; boundaries, quartiles; one-way ANOVA test with Dunn’s multiple comparison). (E and E′) Western blot analysis of VIT-2 protein levels in C. elegans early embryos using an anti-Flag antibody. The band intensity was measured using the “Plot Lanes” function in ImageJ from three independent experiments. The error bars represent 95% CIs (one-way ANOVA test with Dunn’s multiple comparison). (F and F′) Confocal images showing the subcellular localization of SLCF-1-GFP. Statistical analysis was conducted as in B. Source data are available for this figure: SourceData F1.

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