Figure S2.
RAB-10 and EHBP-1 are not involved in apical exocytosis. (A) Statistical analysis data of Fig. S1 is shown as box-and-whisker plots with 10th–90th percentile (n = 24 cells from eight animals of each genotype; dots, outliers; boundaries, quartiles; one-way ANOVA test with Dunn’s multiple comparison). (B and B′) Confocal images showing the distribution of VIT-2-GFP in the C. elegans intestine, oocytes, and embryos in different gene knockdown backgrounds. Statistical data is shown as box-and-whisker plots with 10th–90th percentile (n = 24 cells from eight animals of each genotype; dots, outliers; boundaries, quartiles; one-way ANOVA test with Dunn’s multiple comparison). (C) mRNA level of apoB in different genetic backgrounds in HepG2 cells. Error bars represent 95% CIs (one-way ANOVA test with Dunn’s multiple comparison). (D) Genomic structure of C. elegans ehbp-1 and CRISPR-Cas9 targeting site of ycx123. (E and E′) Confocal images showing EHBP-1-GFP-labeled structures. The statistical data are shown as box-and-whisker plots with the 10th–90th percentile (n = 24 cells from eight animals of each genotype; dots, outliers; boundaries, quartiles; two-tailed Mann-Whitney test). (F and F′) The growth speed of offspring from 1-day-old mothers was measured by their body length at 42 h after hatching. The error bars represent 95% CIs (n = 50 animals; one-way ANOVA test with Dunn’s multiple comparison). (G) The percentage of animals achieving normal development after starvation of L1 larvae. Data were acquired from three independent experiments using 300 animals for each genetic background. Error bars represent 95% CIs (n = 50 animals; one-way ANOVA test with Dunn’s multiple comparison). (H) mRNA level of slcf-1 in different gene knockdown backgrounds in C. elegans. Error bars represent 95% CIs (one-way ANOVA test with Dunn’s multiple comparison). (I and I′) Confocal images showing the subcellular localization of ERM-1-GFP. (J and J′) Confocal images showing the subcellular localization of NHX-2-GFP. Statistical data are shown as box-and-whisker plots with 10th–90th percentile (n = 24 cells from eight animals of each genotype; dots, outliers; boundaries, quartiles; one-way ANOVA test with Dunn’s multiple comparison). (K and K′) Western blot analysis of apoB protein levels in Huh7 cells. Band intensity was measured using the “Plot Lanes” function in ImageJ from three independent experiments. Error bars represent 95% CIs (one-way ANOVA test with Dunn’s multiple comparison). (L and L′) Confocal images showing the subcellular localization of SLCF-1-GFP. Statistical data is shown as box-and-whisker plots with 10th–90th percentile (n = 24 cells from eight animals of each genotype; dots, outliers; boundaries, quartiles; two-way ANOVA with Bonferroni post-test). (M–N′) Confocal images showing the colocalization between VIT-2-mNeonGreen-3xFlagKI and LAAT-1-mCherry or mCherry-GOLG-4. The statistical analysis of colocalization was calculated as the Pearson’s correlation coefficient and is shown as the mean ± SD (n = 18 cells from six animals of each genotype; one-way ANOVA test with Dunn’s multiple comparison). Source data are available for this figure: SourceData FS2. Refer to the image caption for details.

RAB-10 and EHBP-1 are not involved in apical exocytosis. (A) Statistical analysis data of Fig. S1 is shown as box-and-whisker plots with 10th–90th percentile (n = 24 cells from eight animals of each genotype; dots, outliers; boundaries, quartiles; one-way ANOVA test with Dunn’s multiple comparison). (B and B′) Confocal images showing the distribution of VIT-2-GFP in the C. elegans intestine, oocytes, and embryos in different gene knockdown backgrounds. Statistical data is shown as box-and-whisker plots with 10th–90th percentile (n = 24 cells from eight animals of each genotype; dots, outliers; boundaries, quartiles; one-way ANOVA test with Dunn’s multiple comparison). (C) mRNA level of apoB in different genetic backgrounds in HepG2 cells. Error bars represent 95% CIs (one-way ANOVA test with Dunn’s multiple comparison). (D) Genomic structure of C. elegans ehbp-1 and CRISPR-Cas9 targeting site of ycx123. (E and E′) Confocal images showing EHBP-1-GFP-labeled structures. The statistical data are shown as box-and-whisker plots with the 10th–90th percentile (n = 24 cells from eight animals of each genotype; dots, outliers; boundaries, quartiles; two-tailed Mann-Whitney test). (F and F′) The growth speed of offspring from 1-day-old mothers was measured by their body length at 42 h after hatching. The error bars represent 95% CIs (n = 50 animals; one-way ANOVA test with Dunn’s multiple comparison). (G) The percentage of animals achieving normal development after starvation of L1 larvae. Data were acquired from three independent experiments using 300 animals for each genetic background. Error bars represent 95% CIs (n = 50 animals; one-way ANOVA test with Dunn’s multiple comparison). (H) mRNA level of slcf-1 in different gene knockdown backgrounds in C. elegans. Error bars represent 95% CIs (one-way ANOVA test with Dunn’s multiple comparison). (I and I′) Confocal images showing the subcellular localization of ERM-1-GFP. (J and J′) Confocal images showing the subcellular localization of NHX-2-GFP. Statistical data are shown as box-and-whisker plots with 10th–90th percentile (n = 24 cells from eight animals of each genotype; dots, outliers; boundaries, quartiles; one-way ANOVA test with Dunn’s multiple comparison). (K and K′) Western blot analysis of apoB protein levels in Huh7 cells. Band intensity was measured using the “Plot Lanes” function in ImageJ from three independent experiments. Error bars represent 95% CIs (one-way ANOVA test with Dunn’s multiple comparison). (L and L′) Confocal images showing the subcellular localization of SLCF-1-GFP. Statistical data is shown as box-and-whisker plots with 10th–90th percentile (n = 24 cells from eight animals of each genotype; dots, outliers; boundaries, quartiles; two-way ANOVA with Bonferroni post-test). (M–N′) Confocal images showing the colocalization between VIT-2-mNeonGreen-3xFlagKI and LAAT-1-mCherry or mCherry-GOLG-4. The statistical analysis of colocalization was calculated as the Pearson’s correlation coefficient and is shown as the mean ± SD (n = 18 cells from six animals of each genotype; one-way ANOVA test with Dunn’s multiple comparison). Source data are available for this figure: SourceData FS2.

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