VIFs form loose bundle organization compared with keratin. (A) FIB-SEM slice indicating the position of analyzed subvolume presented in Fig. 7 A. (B) Center-aligned average projections of VIFs (left), ribosomes (center), and MTs (right) with the measured size of each averaged structure indicated below. White diagonal lines indicate the position of the line profile used in C. (C) Intensity profile of averaged ribosome, vimentin, and MT profiles shown in C. Plots indicate mean intensity along each profile at 360 1° rotations. (D) Double immunolabeling of a COS-7 cell with anti-vimentin (green) and anti-pan-keratin (magenta) antibodies. (E and F) Immunolabeling of vimentin (E) and keratin 7 (F) in COS-7 cells across a large field of view. Images were collected and stitched together. In F, a stitching artifact is present due to sample drift between sequentially acquired volume tiles. (G and H) VIF bundles from COS-7 cells and (H) keratin bundles from A431 cells highlight the structural dissimilarity between them (source: https://openorganelle.janelia.org/datasets/aic_desmosome-3). (I) Cartoon representation illustrating the loose organization of vimentin bundles versus the highly ordered and compact structure of keratin bundles.