VIFs move independently within bundles. (A) Co-expression of ensemble vimentin reporter (vimentin-mCherry, magenta in overlay) and sparsely labeled vimentin-SunTag (green in overlay). (B) Inset from A showing a vimentin bundle sparsely labeled with vimentin-SunTag. (C) Pseudocolored maximum intensity time projection of vimentin-SunTag particles over 121 frames (60 s). The dashed line indicates the position of the line scan used to generate the kymograph (right). (D) Kymograph reveals uncorrelated movement of individual vimentin-SunTag particles within the observed vimentin bundle.