Vimentin-SunTag aligns and transports along MTs. (A) SIM micrograph of a fixed RPE cell expressing vimentin-SunTag (green) and immunolabeled for endogenous vimentin (magenta) and MTs (blue). (B) RPE cells co-expressing vimentin-SunTag (green) and EB3 (magenta), a snapshot from a time-lapse movie (see Video 8) captured at 2 fps over a 1-min duration. The white box highlights a zoomed-in view of the perinuclear region, showing vimentin-SunTag dots and EB3 comets. (C and D) Pseudocolored maximum intensity projections of EB3 comets and vimentin-SunTag particles from the zoomed-in region. The kymograph generation region for each time sequence is indicated by a blue dotted line, with arrows showing direction.