SPT analysis of vimentin-SunTag reveals equivalent rates of directed VIF motility across cellular regions. (A and B) Frequency distribution of track start positions mapped according to (A) normalized radial position, wherein the nuclear envelope = 0 and the edge of the cell = 1 or (B) distance from cell border in microns. 30,332 trajectories were analyzed, derived from 26 cells. The kernel density estimate is shown as a superimposed line. (C) Schematic indicating spatial mapping used to calculate the distance from the border. (D and E) 2D histograms plotting the initial distance from the cell border and final maximum displacement for either immotile (D) or motile (E) 15-s trajectories.