Figure 3. VIF fractional motility is... | Rockefeller University Press

Figure 3.

$VIF fractional motility is comparable in perinuclear and peripheral regions. (A) Cartoon schematic indicating the radial normalization procedure. Irrespective of the length of the cell along any given angle, the relative position between nuclear envelope (0) and cell border (1) is computed. Perinuclear and peripheral regions are indicated. (B and C) Two-dimensional (2D) histograms plotting the initial normalized radial position and final maximum displacement for either immotile (B) or motile (C) 15-s trajectories. (D) Stacked horizontal bar plot showing the fraction of trajectories in immotile (blue) or motile (red) groups at each of the 10 normalized radial position bins. The fraction of motile trajectories is approximately equal across all radial positions. (E) Normalized radial position map of an RPE cell with motile (>1 µm max. disp. in 15 s) trajectories superimposed. Trajectories are classified as anterograde or retrograde by taking the difference between final and initial normalized radial positions, with positive values classified as anterograde and negative as retrograde. (F) Normalized radial distribution of anterograde motile VIFs and retrograde motile VIFs. Analysis was conducted on a total of 25 individual cells for each condition, collected from three independent experiments. Refer to the image caption for details.$

VIF fractional motility is comparable in perinuclear and peripheral regions. (A) Cartoon schematic indicating the radial normalization procedure. Irrespective of the length of the cell along any given angle, the relative position between nuclear envelope (0) and cell border (1) is computed. Perinuclear and peripheral regions are indicated. (B and C) Two-dimensional (2D) histograms plotting the initial normalized radial position and final maximum displacement for either immotile (B) or motile (C) 15-s trajectories. (D) Stacked horizontal bar plot showing the fraction of trajectories in immotile (blue) or motile (red) groups at each of the 10 normalized radial position bins. The fraction of motile trajectories is approximately equal across all radial positions. (E) Normalized radial position map of an RPE cell with motile (>1 µm max. disp. in 15 s) trajectories superimposed. Trajectories are classified as anterograde or retrograde by taking the difference between final and initial normalized radial positions, with positive values classified as anterograde and negative as retrograde. (F) Normalized radial distribution of anterograde motile VIFs and retrograde motile VIFs. Analysis was conducted on a total of 25 individual cells for each condition, collected from three independent experiments.