Figure S1.
Sparse labeling of vimentin-SunTag in RPE cells. (A) Frequency distribution of fluorescence intensity among detected SunTag particles in a control RPE. (B) Frequency distribution of first NND (microns) between detected SunTag particles in the same cell. (C) Spatial map of vimentin-SunTag localizations, pseudocolored by localization uncertainty (nm) of our fits. (D) Spinning disk confocal micrograph of an RPE cell co-transfected with vimentin-mCherry and vimentin-SunTag (from Fig. 1 B). The ensemble vimentin-mCherry signal is displayed in black, with SunTag localizations overlaid as purple spots. (E) 50 × 50 pixel binned heatmaps of vimentin-mCherry integrated density (left, black) and vimentin-SunTag particle count (right, purple). (F) Scatter plot indicating density of vimentin-mCherry relative to vimentin-SunTag particle detection in each 50 × 50 bin. Spearman’s rank correlation = 0.900. Refer to the image caption for details.

Sparse labeling of vimentin-SunTag in RPE cells. (A) Frequency distribution of fluorescence intensity among detected SunTag particles in a control RPE. (B) Frequency distribution of first NND (microns) between detected SunTag particles in the same cell. (C) Spatial map of vimentin-SunTag localizations, pseudocolored by localization uncertainty (nm) of our fits. (D) Spinning disk confocal micrograph of an RPE cell co-transfected with vimentin-mCherry and vimentin-SunTag (from Fig. 1 B). The ensemble vimentin-mCherry signal is displayed in black, with SunTag localizations overlaid as purple spots. (E) 50 × 50 pixel binned heatmaps of vimentin-mCherry integrated density (left, black) and vimentin-SunTag particle count (right, purple). (F) Scatter plot indicating density of vimentin-mCherry relative to vimentin-SunTag particle detection in each 50 × 50 bin. Spearman’s rank correlation = 0.900.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal